| HIRANUMA APPLICATION DATA | Automatic Titrator | Data No. | B15 | Sep. 12,2018 |
| Drugs and Medicines | Sodium chloride in peritoneal dialysis solution |
1. Abstract
This report introduces an example of measurement for sodium chloride in peritoneal dialysis solution with precipitation titration. The sample is acidified by nitric acid and the concentration of sodium chloride is determined by precipitation titration with silver nitrate titrant using the silver / reference electrode.
NaCl ＋ AgNO₃ → AgCl ＋ NaNO₃
2. Configuration of instruments and reagents
| (1) | Instruments | ||
| Main unit | : | Hiranuma Automatic Titrator COM Series | |
| Electrode | : | Silver combination electrode AGR-811Z (Double Junction Type) *The following electrodes are also usable. ・AGR-801Z (Silver reference combination electrode) ・Combination of AG-311 (Silver indicator electrode) and MS-231Z (Silver reference electrode) ・Combination of AG-311 and RE-241Z (Double junction type silver reference electrode) * Remark The general reference electrode (RE-201Z) cannot be used for this titration because KCl inner solution might come out to sample solution and it causes measurement error. The inner electrodes of AGR-801Z and MS-231Z use mercury (I) sulfate. When these electrodes are disposed, please ask local industrial waste disposal operator. |
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| (2) | Reagents | ||
| Titrant | : | 0.1 mol/L Silver nitrate standard solution | |
| Additive | : | 1 mol/L Nitric acid |
3. Measurement procedure
| (1) | Take 10 ml of sample into 100 mL beaker with volumetric pipette. |
| (2) | Add 40 ml of DI water. |
| (3) | Add 2 ml of 1 mol / L nitric acid. |
| (4) | Immerse the electrodes and titrate with 0.1 mol/L silver nitrate standard solution. |
4. Measurement conditions and results
Examples of titration conditions
Measurement of blank
Measurement of sample
Measurement results
Measurement of blank
| Measurement No. |
Size (mL) |
Titrant volume (mL) |
|---|---|---|
| 1 | – | 0.017 |
| 2 | – | 0.013 |
| 3 | – | 0.015 |
| Statistical result |
Avg. (mL) | 0.015 |
Measurement of sample
| Measurement No. |
Size (mL) |
Titrant volume (mL) |
Sodium chloride (g/L) |
|---|---|---|---|
| 1 | 10 | 10.497 | 6.1318 |
| 2 | 10.490 | 6.1277 | |
| 3 | 10.509 | 6.1388 | |
| Statistical result |
Avg. (g/L) SD (g/L) RSD (%) |
6.133 0.006 0.092 |
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Examples of measurement curves
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5. Note
If the precipitate adheres to the electrode after measurement, please clean it and remove it.
Chloride in peritoneal dialysate solution may contain not only sodium chloride but also calcium chloride and magnesium chloride. Please note that the chloride to be measured is calculated by converting these combined amounts into sodium chloride.
Keywords: Peritoneal dialysate solution, Sodium chloride, Precipitation titration, Silver electrode, Silver nitrate
*Some measurement would not be possible depending on optional configuration of system.
